\u7cbe\u8bfb19 \u2014 \u79fb\u690d\u7684\u4eba\u7c7b\u7eb9\u72b6\u4f53\u7956\u7ec6\u80de\u5728\u4ea8\u5ef7\u987f\u75c5\u52a8\u7269\u6a21\u578b\u4e2d\u7684\u529f\u80fd\u6027\u6574\u5408\n\n> \u6807\u9898: \u79fb\u690d\u7684\u4eba\u7c7b\u7eb9\u72b6\u4f53\u7956\u7ec6\u80de\u5728\u4ea8\u5ef7\u987f\u75c5\u52a8\u7269\u6a21\u578b\u4e2d\u8868\u73b0\u51fa\u529f\u80fd\u6027\u6574\u5408\u5e76\u8c03\u8282\u5bbf\u4e3b\u795e\u7ecf\u56de\u8def\n> \u4f5c\u8005: Linda Scaramuzza, Marta Ribadeneiro, Christian Casarino \u7b49\n> \u673a\u6784: \u610f\u5927\u5229\u7c73\u5170\u5927\u5b66\u3001\u90fd\u7075\u5927\u5b66\u3001\u745e\u5178\u9686\u5fb7\u5927\u5b66\u7b49\n> \u671f\u520a/\u5e74\u4efd: 2025 | 19\u9875\n> \u6587\u732e\u7c7b\u578b: Research Article\n\n---\n\n## \ud83d\udd2c \u7814\u7a76\u80cc\u666f\u4e0e\u76ee\u7684\n\n\u6838\u5fc3\u95ee\u9898: \u4ea8\u5ef7\u987f\u75c5\uff08HD\uff09\u662f\u4e00\u79cd\u9057\u4f20\u6027\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\uff0c\u7279\u5f81\u4e3a\u7eb9\u72b6\u4f53\u4e2d\u578b\u591a\u68d8\u795e\u7ecf\u5143\uff08MSN\uff09\u7684\u8fdb\u884c\u6027\u4e22\u5931\u3002\u76ee\u524d\u7f3a\u4e4f\u6709\u6548\u6cbb\u7597\u65b9\u6cd5\u3002\u672c\u7814\u7a76\u63a2\u7d22\u5c06\u4eba\u7c7b\u80da\u80ce\u5e72\u7ec6\u80de\uff08hESC\uff09\u5206\u5316\u7684\u7eb9\u72b6\u4f53\u7956\u7ec6\u80de\uff08hSPs\uff09\u79fb\u690d\u5230 HD \u5927\u9f20\u6a21\u578b\u4e2d\uff0c\u8bc4\u4f30\u5176\u5b58\u6d3b\u3001\u5206\u5316\u548c\u529f\u80fd\u6574\u5408\u80fd\u529b\u3002\n\n\u5173\u952e\u6311\u6218:\n- HD \u5bfc\u81f4\u7eb9\u72b6\u4f53 GABA \u80fd MSN \u5927\u91cf\u4e22\u5931\n- \u4f20\u7edf\u836f\u7269\u4ec5\u80fd\u7f13\u89e3\u75c7\u72b6\uff0c\u65e0\u6cd5\u4fee\u590d\u795e\u7ecf\u56de\u8def\n- \u5e72\u7ec6\u80de\u79fb\u690d\u9700\u8bc1\u660e\uff1a(1) \u6b63\u786e\u5206\u5316 (2) \u529f\u80fd\u6574\u5408 (3) \u8c03\u8282\u5bbf\u4e3b\u7f51\u7edc\n\n---\n\n## \ud83d\udcca \u5b9e\u9a8c\u8bbe\u8ba1\u4e0e\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\n\n### Figure 1: \u5355\u7ec6\u80de\u6838 RNA \u6d4b\u5e8f\uff08snRNA-seq\uff09\u8868\u5f81\n\n $Figure 1: snRNA-seq \u5206\u6790\u663e\u793a\u79fb\u690d\u7ec6\u80de\u5206\u5316\u4e3a\u591a\u79cd\u7eb9\u72b6\u4f53\u795e\u7ecf\u5143\u7c7b\u578b$ \n\n\u56fe\u6ce8: (a) \u5b9e\u9a8c\u6d41\u7a0b\uff1ahESC \u2192 DIV15 hSPs \u2192 QA \u635f\u4f24\u5927\u9f20\u7eb9\u72b6\u4f53\u79fb\u690d \u2192 21\u5929\u540e\u89e3\u5256\u79fb\u690d\u7269 \u2192 \u5355\u6838\u6d4b\u5e8f\u3002(b) UMAP \u805a\u7c7b\u663e\u793a6\u79cd\u7ec6\u80de\u7c7b\u578b\uff1aCGE\u4e2d\u95f4\u795e\u7ecf\u5143\u3001MGE-LAMP5\u4e2d\u95f4\u795e\u7ecf\u5143\u3001MGE\u6837\u4e2d\u95f4\u795e\u7ecf\u5143\u3001Emerging MSNs\u3001Pre-D1-MSN-like\u3001Pre-D2-MSN-like\u3002(c) \u6807\u8bb0\u57fa\u56e0\u70ed\u56fe\u3002(d) 4\u53ea\u5927\u9f20\u7ec6\u80de\u7ec4\u6210\u6bd4\u4f8b\u3002(e) \u7a7a\u95f4\u5206\u5e03\u6a21\u62df\u3002(f-g) \u4e0e\u6210\u5e74\u5c0f\u9f20\u8111\u56fe\u8c31\u6574\u5408\u9a8c\u8bc1\u3002\n\n\u4e09\u6bb5\u5f0f\u62c6\u89e3:\n\n| \u7ef4\u5ea6 | \u5185\u5bb9 |\n|------|------|\n| \u505a\u4ec0\u4e48 | \u5bf9\u79fb\u690d21\u5929\u540e\u7684\u4eba\u7c7b\u7ec6\u80de\u8fdb\u884c\u5355\u7ec6\u80de\u6838\u6d4b\u5e8f\uff0c\u9274\u5b9a\u5206\u5316\u547d\u8fd0 |\n| \u53d1\u73b0\u4ec0\u4e48 | \u79fb\u690d\u7ec6\u80de\u5206\u5316\u4e3a6\u79cd\u7c7b\u578b\uff1a\u4e2d\u95f4\u795e\u7ecf\u5143\uff08CGE/MGE\u6765\u6e90\uff09+ MSN\u524d\u4f53\uff08D1/D2\u578b\uff09 |\n| \u8bf4\u660e\u4ec0\u4e48 | hSPs \u5728\u4f53\u5185\u80fd\u6b63\u786e\u5206\u5316\u4e3a\u7eb9\u72b6\u4f53\u4e3b\u8981\u795e\u7ecf\u5143\u7c7b\u578b\uff0c\u4e0e\u6210\u5e74 MSN \u57fa\u56e0\u8868\u8fbe\u9ad8\u5ea6\u76f8\u5173 |\n\n---\n\n## \ud83d\udcca \u4f53\u5185\u79fb\u690d\u8868\u5f81\n\n### Figure 2: \u514d\u75ab\u7ec4\u5316\u9a8c\u8bc1\u7ec6\u80de\u5206\u5316\n\n $Figure 2: \u79fb\u690d\u7ec6\u80de\u5728\u4f53\u5185\u5206\u5316\u4e3a\u7eb9\u72b6\u4f53\u795e\u7ecf\u5143$ \n\n\u56fe\u6ce8: (a) \u5b9e\u9a8c\u8bbe\u8ba1\uff1a3\u6279\u6b21 hESC \u2192 hSPs \u2192 QA \u5927\u9f20\u79fb\u690d \u2192 \u8111\u5207\u7247\u514d\u75ab\u7ec4\u5316\u3002(b-c) \u5b9a\u91cf\uff1a55% HuNu+ \u7ec6\u80de\u8868\u8fbe CTIP2\uff08\u795e\u7ecf\u5143\uff09\uff0c30% \u8868\u8fbe SOX9\uff08\u80f6\u8d28\uff09\uff1b80% CTIP2+ \u7ec6\u80de\u4e3a GABA \u80fd\uff0c15-20% \u8868\u8fbe DARPP32\uff08MSN \u6807\u8bb0\uff09\u3002(d-i) \u514d\u75ab\u8367\u5149\u663e\u793a pENK\uff08\u95f4\u63a5\u901a\u8def\uff09\u3001SubP\uff08\u76f4\u63a5\u901a\u8def\uff09\u3001DCX\uff08\u8fc1\u79fb\uff09\u3001SOX9/SOX10\uff08\u80f6\u8d28\uff09\u6807\u8bb0\u3002(j) 2MPT vs 6MPT \u65f6\u95f4\u8fdb\u7a0b\u6bd4\u8f83\u3002\n\n\u4e09\u6bb5\u5f0f\u62c6\u89e3:\n\n| \u7ef4\u5ea6 | \u5185\u5bb9 |\n|------|------|\n| \u505a\u4ec0\u4e48 | \u514d\u75ab\u7ec4\u5316\u5206\u6790\u79fb\u690d\u540e2\u4e2a\u6708\u548c6\u4e2a\u6708\u7684\u7ec6\u80de\u5206\u5316\u8868\u578b |\n| \u53d1\u73b0\u4ec0\u4e48 | 55% \u5206\u5316\u4e3a CTIP2+ \u795e\u7ecf\u5143\uff0c80% \u4e3a GABA \u80fd\uff0c15-20% \u8868\u8fbe DARPP32\uff1b\u540c\u65f6\u5206\u5316\u51fa\u76f4\u63a5\u901a\u8def\uff08SubP+\uff09\u548c\u95f4\u63a5\u901a\u8def\uff08pENK+\uff09MSN |\n| \u8bf4\u660e\u4ec0\u4e48 | \u79fb\u690d\u7ec6\u80de\u4e0d\u4ec5\u5b58\u6d3b\uff0c\u8fd8\u6b63\u786e\u5206\u5316\u4e3a\u7eb9\u72b6\u4f53\u4e24\u79cd\u529f\u80fd\u4e9a\u578b\uff0c\u4e14\u968f\u65f6\u95f4\u6210\u719f\uff08GABA \u8868\u8fbe\u4ece2M\u52306M\u663e\u8457\u589e\u52a0\uff09 |\n\n---\n\n## \ud83d\udcca \u529f\u80fd\u6574\u5408\u9a8c\u8bc1\n\n### Figure 4: \u5149\u7ea4\u5149\u5ea6\u6cd5\u8bb0\u5f55\u9499\u6d3b\u52a8\n\n $Figure 4: \u79fb\u690d\u795e\u7ecf\u5143\u8868\u73b0\u51fa\u72b6\u6001\u4f9d\u8d56\u6027\u548c\u523a\u6fc0\u54cd\u5e94\u6027\u9499\u6d3b\u52a8$ \n\n\u56fe\u6ce8: (a) \u5b9e\u9a8c\u8bbe\u8ba1\uff1ahSPs \u8f6c\u67d3 jGCaMP7f\uff08\u9499\u6307\u793a\u5242\uff09\u2192 \u79fb\u690d\u5230 QA \u5927\u9f20 \u2192 \u690d\u5165\u5149\u7ea4\u8bb0\u5f55\u3002(b) \u7ec4\u7ec7\u5b66\u9a8c\u8bc1\uff1ajGCaMP7f\uff08\u7eff\uff09\u4e0e NeuN\uff08\u7ea2\uff09\u5171\u5b9a\u4f4d\u3002(c-f) \u81ea\u53d1\u6d3b\u52a8\uff1a\u6d3b\u8dc3\u6e05\u9192\uff08AW\uff09\u72b6\u6001\u9499\u77ac\u53d8\u9891\u7387\u548c\u632f\u5e45\u663e\u8457\u9ad8\u4e8e\u9ebb\u9189\uff08AN\uff09\u72b6\u6001\u3002(g-j) \u8bf1\u53d1\u7535\u4f4d\uff1a\u8db3\u5e95\u7535\u51fb\uff08FSK\uff09\u540e jGCaMP7f \u79fb\u690d\u7ec4\u4fe1\u53f7\u663e\u8457\u589e\u5f3a\uff0cGFP \u5bf9\u7167\u7ec4\u65e0\u53cd\u5e94\u3002\n\n\u4e09\u6bb5\u5f0f\u62c6\u89e3:\n\n| \u7ef4\u5ea6 | \u5185\u5bb9 |\n|------|------|\n| \u505a\u4ec0\u4e48 | \u7528\u5149\u7ea4\u5149\u5ea6\u6cd5\u8bb0\u5f55\u79fb\u690d\u540e6-10\u4e2a\u6708\u7684\u4eba\u7c7b\u795e\u7ecf\u5143\u9499\u6d3b\u52a8 |\n| \u53d1\u73b0\u4ec0\u4e48 | (1) \u6e05\u9192\u72b6\u6001\u9ad8\u9891\u9499\u77ac\u53d8\uff0c\u9ebb\u9189\u72b6\u6001\u51e0\u4e4e\u65e0\u6d3b\u52a8\uff1b(2) \u8db3\u5e95\u7535\u51fb\u540e\u9499\u4fe1\u53f7\u663e\u8457\u589e\u5f3a\uff0c\u5bf9\u7167\u7ec4\u65e0\u53cd\u5e94 |\n| \u8bf4\u660e\u4ec0\u4e48 | \u79fb\u690d\u795e\u7ecf\u5143\u4e0d\u4ec5\u5b58\u6d3b\uff0c\u8fd8\u529f\u80fd\u6027\u6574\u5408\u5230\u5bbf\u4e3b\u795e\u7ecf\u7f51\u7edc\u4e2d\uff0c\u80fd\u63a5\u6536\u5e76\u5904\u7406\u5916\u754c\u523a\u6fc0\u4fe1\u53f7 |\n\n---\n\n## \ud83d\udca1 \u79d1\u5b66\u610f\u4e49\u4e0e\u4ea7\u54c1\u5173\u8054\n\n### 1. \u5e72\u7ec6\u80de\u6cbb\u7597 HD \u7684\u7a81\u7834\n\n| \u7ef4\u5ea6 | \u4f20\u7edf\u65b9\u6cd5 | \u672c\u7814\u7a76 |\n|------|---------|--------|\n| \u6cbb\u7597\u7b56\u7565 | \u75c7\u72b6\u7f13\u89e3\uff08\u591a\u5df4\u80fa\u8c03\u8282\uff09 | \u795e\u7ecf\u56de\u8def\u4fee\u590d |\n| \u7ec6\u80de\u6765\u6e90 | \u80ce\u513f\u7ec4\u7ec7\uff08\u6709\u9650\uff09 | hESC \u4f53\u5916\u5206\u5316\uff08\u53ef\u6269\u5c55\uff09 |\n| \u529f\u80fd\u9a8c\u8bc1 | \u884c\u4e3a\u5b66\u6539\u5584 | \u9499\u4fe1\u53f7\u8bb0\u5f55\u8bc1\u660e\u7f51\u7edc\u6574\u5408 |\n| \u5206\u5316\u7cbe\u5ea6 | \u6df7\u5408\u7ec6\u80de\u7fa4 | D1/D2 MSN + \u4e2d\u95f4\u795e\u7ecf\u5143\u591a\u4e9a\u578b |\n\n### 2. \u5173\u952e\u6280\u672f\u5e73\u53f0\n\n| \u6280\u672f | \u5e94\u7528 | \u6f5c\u5728\u5ba2\u6237 |\n|------|------|---------|\n| \u5355\u7ec6\u80de\u6838 RNA \u6d4b\u5e8f | \u79fb\u690d\u7ec6\u80de\u5206\u5316\u547d\u8fd0\u9274\u5b9a | \u9ad8\u6821\u3001\u7814\u7a76\u6240\u3001\u836f\u4f01 |\n| \u5149\u7ea4\u5149\u5ea6\u6cd5 | \u4f53\u5185\u9499\u6d3b\u52a8\u8bb0\u5f55 | \u795e\u7ecf\u79d1\u5b66\u5b9e\u9a8c\u5ba4 |\n| QA \u635f\u4f24\u5927\u9f20\u6a21\u578b | \u4ea8\u5ef7\u987f\u75c5\u52a8\u7269\u6a21\u578b | \u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u7814\u7a76 |\n| \u7acb\u4f53\u5b9a\u4f4d\u6ce8\u5c04 | \u7cbe\u786e\u8111\u533a\u79fb\u690d | \u795e\u7ecf\u5916\u79d1\u3001\u5e72\u7ec6\u80de\u6cbb\u7597 |\n\n### 3. \u4ea7\u54c1\u5173\u8054\n\n| \u4ea7\u54c1/\u6280\u672f | \u5e94\u7528\u573a\u666f | \u661f\u7ea7 |\n|-----------|---------|------|\n| \u5149\u7ea4\u5149\u5ea6\u6cd5\u7cfb\u7edf | \u4f53\u5185\u795e\u7ecf\u5143\u6d3b\u52a8\u8bb0\u5f55 | \u2b50\u2b50\u2b50\u2b50\u2b50 |\n| \u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f0 | \u7ec6\u80de\u5206\u5316\u8868\u5f81 | \u2b50\u2b50\u2b50\u2b50 |\n| \u7acb\u4f53\u5b9a\u4f4d\u4eea | \u7cbe\u786e\u8111\u533a\u79fb\u690d | \u2b50\u2b50\u2b50\u2b50 |\n| \u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf | \u79fb\u690d\u7ec6\u80de\u8ffd\u8e2a | \u2b50\u2b50\u2b50 |\n\n---\n\n## \ud83d\udcf8 \u914d\u56fe\n\n### \u914d\u56fe1: snRNA-seq \u7ec6\u80de\u805a\u7c7b\n\n $Figure 1: UMAP \u805a\u7c7b\u663e\u793a6\u79cd\u79fb\u690d\u7ec6\u80de\u7c7b\u578b$ \n\n\u56fe\u6ce8: \u5355\u7ec6\u80de\u6838\u8f6c\u5f55\u7ec4\u5206\u6790\u9274\u5b9a\u79fb\u690d\u7ec6\u80de\u5206\u5316\u4e3a CGE \u4e2d\u95f4\u795e\u7ecf\u5143\u3001MGE-LAMP5 \u4e2d\u95f4\u795e\u7ecf\u5143\u3001Emerging MSNs\u3001Pre-D1-MSN-like\u3001Pre-D2-MSN-like \u7b496\u79cd\u7c7b\u578b\uff0c\u4e0e\u6210\u5e74\u5c0f\u9f20\u8111\u56fe\u8c31\u9ad8\u5ea6\u76f8\u5173\u3002\n\n### \u914d\u56fe2: \u4f53\u5185\u514d\u75ab\u7ec4\u5316\u8868\u5f81\n\n $Figure 2: \u79fb\u690d\u7ec6\u80de\u5206\u5316\u4e3a\u7eb9\u72b6\u4f53\u795e\u7ecf\u5143$ \n\n\u56fe\u6ce8: \u514d\u75ab\u8367\u5149\u663e\u793a 55% \u79fb\u690d\u7ec6\u80de\u8868\u8fbe CTIP2\uff08\u795e\u7ecf\u5143\uff09\uff0c80% \u4e3a GABA \u80fd\uff0c15-20% \u8868\u8fbe DARPP32\uff08MSN \u7279\u5f02\u6027\u6807\u8bb0\uff09\uff0c\u5e76\u5206\u5316\u51fa\u76f4\u63a5\u901a\u8def\uff08SubP+\uff09\u548c\u95f4\u63a5\u901a\u8def\uff08pENK+\uff09\u4e9a\u578b\u3002\n\n### \u914d\u56fe3: \u5149\u7ea4\u5149\u5ea6\u6cd5\u529f\u80fd\u9a8c\u8bc1\n\n $Figure 4: \u79fb\u690d\u795e\u7ecf\u5143\u9499\u6d3b\u52a8\u8bb0\u5f55$ \n\n\u56fe\u6ce8: \u5149\u7ea4\u5149\u5ea6\u6cd5\u8bb0\u5f55\u663e\u793a\u79fb\u690d\u795e\u7ecf\u5143\u5728\u6e05\u9192\u72b6\u6001\u8868\u73b0\u51fa\u9ad8\u9891\u9499\u77ac\u53d8\uff0c\u5bf9\u8db3\u5e95\u7535\u523a\u6fc0\u4ea7\u751f\u663e\u8457\u54cd\u5e94\uff0c\u8bc1\u660e\u529f\u80fd\u6027\u6574\u5408\u5230\u5bbf\u4e3b\u795e\u7ecf\u7f51\u7edc\u3002\n\n---\n\n## \ud83d\udcd6 \u53c2\u8003\u6587\u732e\n\n1. Scaramuzza L, et al. Transplanted human striatal progenitors exhibit functional integration and modulate host circuitry in a Huntington's disease animal model. 2025.\n2. \u4ea8\u5ef7\u987f\u75c5\u5e72\u7ec6\u80de\u6cbb\u7597\u9886\u57df\u76f8\u5173\u6587\u732e\uff08QA \u635f\u4f24\u6a21\u578b\u3001MSN \u5206\u5316\u534f\u8bae\u3001\u5149\u7ea4\u5149\u5ea6\u6cd5\u7b49\uff09\n\n---\n\n\u8054\u7cfb\u4eba\uff5c\u674e\u4e1c\u4f1f\n\ud83d\udcde 18045130660\n\ud83c\udf10 \u4e3b\u9875\uff1ahttps://www.biokingdom.top\n