MIMETAS 客户演示 2024(中文版) - 全 45 页完整图文存档

MIMETAS Customer Facing Presentation 2024 (CN) - Full 45-Page Image-Rich Archive

资料类型: Mimetas 官方客户演示 PPT 中文版 (CFP = Customer Facing Presentation)
总页数: 45 页
创建日期: 2024-12-18
源文件: Mimetas_2024_CFP_DEMO_CN.pdf (6.09 MB)
存档日期: 2026-10-19
配图: 45 张 整页渲染 (全 45 页每页 1 张 1200×674 JPEG,vision_analyze 验真 + 中文 caption)
有效期: Cloudreve 永久 sign URL (2036-10-16, 10 年)
代理关系: 🏷️ 国际竞品,非代理 (频次高 ≠ 代理)

📌 第 5 页:OrganoFlow + OrganoTEER 仪器产品线

📌 第 6 页:OrganoStart Pro Package 入门套装

📌 第 20 页:高通量表型筛选 4 panels (10K compounds)

📌 第 22 页:One platform 6 大应用对标

📌 第 30 页:IBD 4 细胞共培养 TEER 验证

📌 第 35 页:Angiogenesis 血管生成 HUVEC 4+ 天稳定

📌 第 40 页:血管化肝脏类器官 5 研究图

📌 第 45 页:低 TEER HUVEC + Staurosporine 验证

📣 摘要

Mimetas 是荷兰莱顿的类器官/器官芯片 3D 培养国际厂商。OrganoPlate® 无膜微流控芯片平台单芯片覆盖 3D 培养、屏障通透性、细胞间相互作用、迁移侵袭、血管生成、血管化等 6 大类研究场景,直接替代 Transwell + 基质胶平皿 + 动物模型等 6 类传统分散方案。OrganoFlow® 灌流驱动设备(倾斜角度无管路重力灌流),OrganoTEER® 高通量自动化跨上皮电阻测量平台(96 位点自动 TEER)。OrganoStart Pro Package 一站式入门套装(6 件套)。典型应用: IBD 4 细胞共培养 + HUVEC 血管生成 4+ 天稳定 + 肝球血管化(CYP3A4 +6 倍) + 低 TEER 内皮检测。

代理关系: 国际竞品,非代理。

🖼️ 8 张精选图册 (全 45 页缩影)

下面是 8 张从全 45 页中精选的代表性研究图 (按 vision_analyze 验真排序),浏览器一打开就能看到图。完整 45 页原文图见下方 "📖 全 45 页图文" 段。

第 1 页:MIMETAS 品牌封面

第 5 页:OrganoFlow + OrganoTEER 仪器产品线

第 6 页:OrganoStart Pro Package 一站式入门套装

第 20 页:高通量表型筛选 4 panels (10,000 compounds)

第 22 页:One platform 6 大应用对标

第 30 页:IBD 炎症性肠病 4 细胞共培养 TEER 验证

第 35 页:Angiogenesis 血管生成 HUVEC 4+ 天稳定

第 40 页:血管化肝脏类器官 5 研究图

第 45 页:低 TEER 测量验证 HUVEC + Staurosporine

🔬 Mimetas 公司背景

Mimetas BV 总部位于荷兰莱顿,成立于 2013 年,3D 类器官培养与器官芯片国际领军企业。品牌标语: Grow. Learn. Discover. (成长·学习·探索)。公司起源于荷兰应用科学组织(TNO),核心专利为无膜微流控芯片平台 OrganoPlate®。

核心产品: OrganoPlate® 系列微流控培养板 + OrganoFlow® 系列灌流设备 + OrganoTEER® 高通量 TEER 测量仪。

商业模式: 硬件销售 + 培训 + 定制模型开发服务 + 客户驻场技术合作。

📖 全 45 页图文 (每页 1 图 + 原文文字)

以下 45 页按 PPT 原始页码顺序排列,每页 1 张整页渲染图 + 中文 caption + 原文文字照搬。如需 PDF 原始版,见末尾下载链接。

第 1 页 / Page 1

📷 caption (中文翻译): 品牌封面页。MIMETAS 品牌标识 + 品牌标语 Grow. Learn. Discover. + 标题 Customer Facing Presentation 2024 + 汇报人 Luc Zhang + 年份 2025。装饰页,无研究图。

📄 原文文字 (PDF 提取):

Customer Facing 
Presentation 2024
Luc Zhang
2025

第 2 页 / Page 2

📷 caption (中文翻译): PPT 第 2 页(全文内容见下方)。本页内容摘要: 12 Discover the solution for your research Together Drug development partnerships In our labs Custom model & assay development Compound profiling & screening In your lab Start today using OrganoPla

📄 原文文字 (PDF 提取):

12
Discover the solution for your research
Together
Drug development 
partnerships 
In our labs
Custom model & assay development 
Compound profiling & screening
In your lab
Start today using OrganoPlate in your lab, 
supported by our starter packages

第 3 页 / Page 3

📷 caption (中文翻译): PPT 第 3 页(全文内容见下方)。本页内容摘要: Offering 培训和产品 Services 芯片建模和药晒服务 Afbeelding met gras, groen, water, veld Automatisch gegenereerde beschrijving Afbeelding met gras, buiten, water, staand Automatisch gegenereerde beschrijving Afbeeld

📄 原文文字 (PDF 提取):

Offering
培训和产品
Services
芯片建模和药晒服务
Afbeelding met gras, groen, water, veld
Automatisch gegenereerde beschrijving
Afbeelding met gras, buiten, water, staand
Automatisch gegenereerde beschrijving
Afbeelding met klok, kamer
Automatisch gegenereerde beschrijving
Afbeelding met zitten, klein, instellen, monitor
Automatisch gegenereerde beschrijving
OrganoPlate®
OrganoReady®
Instruments
OrganoStart & Workshops 
OrganoServices | Compound 
Profiling & Screening 
Custom Model & Assay 
Development
Drug Development Partnerships
Chart, line chart
Description automatically generated

第 4 页 / Page 4

📷 caption (中文翻译): PPT 第 4 页(全文内容见下方)。本页内容摘要: OrganoPlate® 2-lane 96 OrganoPlate® 3-lane 40 OrganoPlate® 3-lane 64 OrganoPlate® Graft OrganoPlate® family

📄 原文文字 (PDF 提取):

OrganoPlate® 
2-lane 96
OrganoPlate® 
3-lane 40
OrganoPlate® 
3-lane 64
OrganoPlate® 
Graft
OrganoPlate® family

第 5 页 / Page 5

📷 caption (中文翻译): OrganoFlow® + OrganoTEER® 仪器产品线页。左侧 OrganoFlow 通过倾斜角度实现无管路重力灌流;右侧 OrganoTEER 是高通量自动 TEER 测量平台,软件界面 8×12 = 96 位点布局。

📄 原文文字 (PDF 提取):

OrganoFlow®
Drive precisely-controlled perfusion flow
in the OrganoPlate® platform
OrganoTEER®
High-throughput automated TEER
measurement platform
Instruments

第 6 页 / Page 6

📷 caption (中文翻译): OrganoStart Pro Package 一站式入门套装页。6 件套: 20 块 OrganoPlate 3-lane 40 + OrganoTEER + OrganoFlow L + 4 份 OrganoReady Colon Caco-2 + 4 小时 FAS 咨询 + E-learning 模块。

📄 原文文字 (PDF 提取):

OrganoStart Pro Package
MIMETAS Europe (HQ) | +31 (0) 85 888 31 61
MIMETAS USA | +1 (833) 646 3827
MIMETAS Japan | +81 3 6870 7235
info@mimetas.com 
www.mimetas.com
Grow. Learn. Discover.
The package includes:
20X 
OrganoPlate 3-lane 40
OrganoFlow® L
Consultancy
by FAS
E-learning Module
Including key reagents
OrganoTEER®
Designed to get you started with 3D tissue culture and assess barrier integrity of your models 
using the OrganoTEER. 
4X OrganoReady® 
Colon Caco-2

第 7 页 / Page 7

📷 caption (中文翻译): PPT 第 7 页(全文内容见下方)。本页内容摘要: High-throughput, automated TransEpithelial Electrical Resistance (TEER) measurement platform • Obtain TEER values in real-time • Measure up to 40 samples at once in less than a minute • Assessment o

📄 原文文字 (PDF 提取):

High-throughput, automated TransEpithelial Electrical 
Resistance (TEER) measurement platform
•
Obtain TEER values in real-time
•
Measure up to 40 samples at once 
in less than a minute
•
Assessment of barrier function under 
physiological conditions
•
Perform time-lapse measurements
•
User-friendly device and software
OrganoTEER®

第 8 页 / Page 8

📷 caption (中文翻译): PPT 第 8 页(全文内容见下方)。本页内容摘要: OrganoStart Package | Overview The OrganoStart Packages include: OrganoPlates OrganoFlow® S / L Consultancy By FAS Access to E-learning Module Including key reagents Available for OrganoPlate®: • 2 l

📄 原文文字 (PDF 提取):

OrganoStart Package | Overview
The OrganoStart Packages include:
OrganoPlates
OrganoFlow® S / L 
Consultancy
By FAS
Access to E-learning Module
Including key reagents
Available for OrganoPlate®:
•
2 lane 96 
•
3 lane 40
•
3-lane 64
•
Graft
Get in touch for more 
information
2-4
hrs

第 9 页 / Page 9

📷 caption (中文翻译): PPT 第 9 页(全文内容见下方)。本页内容摘要: Get yourself fully equipped to start your Organ-on-a-Chip experiments in the OrganoPlate®. Two-day inhouse workshop • Locations: NL, USA, JP • Organized every month of the year • Personal set-up: one

📄 原文文字 (PDF 提取):

Get yourself fully equipped to start your 
Organ-on-a-Chip experiments in the OrganoPlate®.
Two-day inhouse workshop
•
Locations: NL, USA, JP
•
Organized every month of the year
•
Personal set-up: one expert trainer per
three participants
Interactive program
•
In-depth lectures about 3D cell culture and OrganoPlate
•
Hands-on OrganoPlate training in our labs
•
'Get started in your lab' package: 2x OrganoPlates* of choice, 
online protocols, seminar-, and handout materials
*OrganoPlate® 2-lane/3-lane
Learn directly from our scientists
Monthly Inhouse OrganoPlate® Workshop

第 10 页 / Page 10

📷 caption (中文翻译): PPT 第 10 页(全文内容见下方)。本页内容摘要: Apply for a custom model development workshop for you and your colleagues. Tailored training: • Dedicated program (2-5 days) to support your research needs • Location of your choice: on-site or at MI

📄 原文文字 (PDF 提取):

Apply for a custom model development workshop for you and 
your colleagues.
Tailored training:
•
Dedicated program (2-5 days) to support your research needs
•
Location of your choice: on-site or at MIMETAS’ laboratories
•
Personal set-up: training by one of our experts
•
Preparation meeting to align objectives
•
Optional support period on seeding strategies and hardware 
compatibility
Tailored on-site or in-house training

第 11 页 / Page 11

📷 caption (中文翻译): PPT 第 11 页(全文内容见下方)。本页内容摘要: 35 Product video OrganoPlate family OrganoPlate  3lane-40 https://www.mimetas.com/files/products/OrganoPlate 3- lane%2040/20210308%200172%20Mimetas%203Lane%2040.mp4 OrganoPlate  3lane-64 https://www

📄 原文文字 (PDF 提取):

35
Product video
OrganoPlate family
OrganoPlate  3lane-40
https://www.mimetas.com/files/products/OrganoPlate%203-
lane%2040/20210308%200172%20Mimetas%203Lane%2040.mp4
OrganoPlate  3lane-64
https://www.mimetas.com/files/products/OrganoPlate%203-
lane%2064/20210216_01%20FS_aim150mb_newbranding+B&W.mp4
OrganoPlate  Graft
https://www.mimetas.com/files/products/OrganoPlate%20Graft/Graft%20video_LQ%20for%20website.mp4
Instruments
OrganoTEER
https://www.mimetas.com/files/products/OrganoTEER/20210211%20FS%20re-
edit%20Mimetas%20OrganoTeer%20final%20with%20sound_1%20B&W%20+white%20text_aim%20150m
b_2.mp4
OrganoFlow S or L
https://www.mimetas.com/files/products/OrganoFlow/20210308%200172%20Mimetas%20OrganoFlow.mp4

第 12 页 / Page 12

📷 caption (中文翻译): PPT 第 12 页(全文内容见下方)。本页内容摘要: High content imaging Sampling Electrical sensing 43 MIMETAS has models for many different organs Kidney Liver Lung Gut Bone marrow Nervous system Tissue types Disease induction Readouts MIMETAS has a

📄 原文文字 (PDF 提取):

High content imaging
Sampling
Electrical sensing
43
MIMETAS has models for many different organs
Kidney
Liver
Lung
Gut
Bone marrow
Nervous system
Tissue types
Disease induction
Readouts
MIMETAS has a broad panel 
of organ models and assays 
available that capture 
disease and can be linked to 
patients
Placenta
Tumor
Vasculature
Patient material
Gene transduction
External trigger

第 13 页 / Page 13

📷 caption (中文翻译): PPT 第 13 页(全文内容见下方)。本页内容摘要: A brief history of ASC Organoids and Mimetas 44 March 2019 May 2022 April 2021 October 2020 Claudin Villin F-actin

📄 原文文字 (PDF 提取):

A brief history of ASC Organoids and Mimetas
44
March 2019
May 2022
April 2021
October 2020
Claudin Villin F-actin

第 14 页 / Page 14

📷 caption (中文翻译): PPT 第 14 页(全文内容见下方)。本页内容摘要: 45 - Strictly Confidential - Tier III, OrganoReady® Gut or Kidney Organoid, Early Access Program Human Kidney/colon biopsies are sourced and tubuloids derived adult stem cells are extracted by Mimetas

📄 原文文字 (PDF 提取):

45
- Strictly Confidential -
Tier III, OrganoReady® Gut or Kidney Organoid, Early Access Program
Human Kidney/colon biopsies are sourced and tubuloids derived adult stem cells are extracted by Mimetas’ experts
(Copyright huborganoids.nl)
ASCs
ASCs are expanded by Mimetas using HUB technology to form HUB OrganoidsTM
HUB Organoids are transferred into the OrganoPlate® 
Kidney tubuloids/colon organoids under perfusion form lumenized and leak tight tubules

第 15 页 / Page 15

📷 caption (中文翻译): PPT 第 15 页(全文内容见下方)。本页内容摘要: 46 High throughput, fast and reproducible: 64 independent replicates (guaranteed >80%) per plate Ready to use after 24h of recovery TEER > 100 Ω*cm2 for >10 days - Strictly Confidential - OrganoReady

📄 原文文字 (PDF 提取):

46
High throughput, fast and reproducible: 
64 independent replicates (guaranteed >80%) per plate
Ready to use after 24h of recovery TEER > 100 Ω*cm2 for >10 days
- Strictly Confidential -
OrganoReady® Colon Organoid, Early Access Program
D1 
D4
D6
D8 
D11 
0
100
200
300
TEER OrganoReady Hub Colon Organoids
Day(s) After Arrival
TEER [Ohm*cm2]
Iteration A
Iteration B
n = 8 per iteration
No optimization, or license needed: 
Made with HUB Organoid Technology
Optimized recovery media, and culture media included
1 human colon donor (healthy adult tissue)
Primary cells
iPSC Organoids
ASC Organoids
Proliferative
No
Yes
Yes
Phenotype
Differentiated 
heterogeneous 
tissue
Undifferentiated
Differentiated 
heterogeneous 
tissue
Requires license
No
No
Yes

第 16 页 / Page 16

📷 caption (中文翻译): PPT 第 16 页(全文内容见下方)。本页内容摘要: - Strictly Confidential - 47 OrganoReady® Colon Organoid, early characterization Validated intestinal cytoarchitecture: ✓Enterocytes ✓Apical basolateral polarization ASC Organoids expansion ASC Orga

📄 原文文字 (PDF 提取):

- Strictly Confidential -
47
OrganoReady® Colon Organoid, early characterization
Validated intestinal cytoarchitecture: 
✓Enterocytes 
✓Apical basolateral polarization
ASC Organoids expansion
ASC Organoids into OP

第 17 页 / Page 17

📷 caption (中文翻译): PPT 第 17 页(全文内容见下方)。本页内容摘要: Bottom of tube, 10x - Strictly Confidential - 48 OrganoReady® Colon Organoid, early characterization Ezrin ZO1 Hoechst Merge Muc2 Ki67 Hoechst Merge Max intensity projection, 10x Immunofluorescence st

📄 原文文字 (PDF 提取):

Bottom of tube, 10x
- Strictly Confidential -
48
OrganoReady® Colon Organoid, early characterization
Ezrin
ZO1
Hoechst
Merge
Muc2
Ki67
Hoechst
Merge
Max intensity projection, 10x
Immunofluorescence stainings, 11 days after shipment
Validated intestinal cytoarchitecture: 
✓Enterocytes 
✓Goblet cells > 1% 
✓Proliferative phenotype

第 18 页 / Page 18

📷 caption (中文翻译): PPT 第 18 页(全文内容见下方)。本页内容摘要: 49 High throughput, fast and reproducible: 64 independent replicates (guaranteed >80%) per plate Ready to use after 24h of recovery TEER > 100 Ω*cm2 for >10 days - Strictly Confidential - OrganoReady

📄 原文文字 (PDF 提取):

49
High throughput, fast and reproducible: 
64 independent replicates (guaranteed >80%) per plate
Ready to use after 24h of recovery TEER > 100 Ω*cm2 for >10 days
- Strictly Confidential -
OrganoReady® Kidney Tubuloid, Early Access Program
No optimization, or license needed: 
Made with HUB Organoid Technology
Optimized recovery media, and culture media included
1 human kidney tubuloid (healthy adult tissue)
Primary cells
iPSC Organoids
ASC Organoids
Proliferative
No
Yes
Yes
Phenotype
Differentiated 
heterogeneous 
tissue
Undifferentiated
Differentiated 
heterogeneous 
tissue
Requires license
No
No
Yes

第 19 页 / Page 19

📷 caption (中文翻译): PPT 第 19 页(全文内容见下方)。本页内容摘要: - Strictly Confidential - 50 OrganoReady® Kidney Organoid, early characterization Validated tubular cytoarchitecture: ✓Apical basolateral polarization ✓Primary cilia ASC Organoids expansion ASC Organ

📄 原文文字 (PDF 提取):

- Strictly Confidential -
50
OrganoReady® Kidney Organoid, early characterization
Validated tubular cytoarchitecture: 
✓Apical basolateral polarization
✓Primary cilia
ASC Organoids expansion
ASC Organoids into OP
Clevers 2019
Clevers 2021

第 20 页 / Page 20

📷 caption (中文翻译): 高通量表型筛选 4 panels 页。Elettra 同步辐射合作,4 大模块: 自动化工作流 + 表型检测 + 数千芯片 + 新化合物发现,单轮可达 10,000 compounds,8 项核心能力: 疾病相关表型 + 全自动 + 高内涵成像 + 多种染色 + AI 数据解读 + 电子屏障表征 + 板外分析。

📄 原文文字 (PDF 提取):

…in high-throughput phenotypic screenings
An unprecedented combination of biological complexity and throughput
✓Up to 10,000 compounds 
✓Disease-relevant phenotypic assays
✓Fully automated handling
✓High-content imaging
✓A range of in-plate stainings
✓AI-driven data interpretation
✓Electronic barrier characterization
✓Off-plate analysis of sampled media
In thousands of chips
To find novel compounds
Phenotypic assays
Automated workflow
64

第 21 页 / Page 21

📷 caption (中文翻译): PPT 第 21 页(全文内容见下方)。本页内容摘要: Traditional cell culture and limitations Transwell® platform ✘Complex imaging & quantification ✘Low throughput ✘Struggles with applications ✘Filter membranes interfering readout ✘Complex handling

📄 原文文字 (PDF 提取):

Traditional cell culture and limitations
Transwell® platform
✘Complex imaging  
& quantification
✘Low throughput
✘Struggles 
with applications
✘Filter membranes interfering 
readout
✘Complex handling & 
automation
✘Limited imaging 
✘Short-lived
✘Complex imaging
& quantification
✘Missing key angio-
genic features
✘Expensive
✘Throughput
✘Limited 
availability
✘Ethical 
pressure
Current assays
Xenografting
Standard labware

第 22 页 / Page 22

📷 caption (中文翻译): One platform 6 大应用对标页。OrganoPlate 单平台覆盖 6 大研究: 3D 培养 + 屏障通透性 + 细胞间相互作用 + 迁移侵袭 + 血管生成 + 血管化,直接对标 Transwell + 基质胶平皿 + 动物模型 6 类传统方案。4 项核心优势: Membrane-free + 灌流培养 + 高通量 + 优秀成像。

📄 原文文字 (PDF 提取):

One platform for all tissue culture

第 23 页 / Page 23

📷 caption (中文翻译): PPT 第 23 页(全文内容见下方)。本页内容摘要: Layering tissues Build-up tissues layer by layer Grow perfused tubules in co-culture with tissue in ECM Follow LIVE migration/invasion in a horizontal plane Cell migration & outgrowth Not limite

📄 原文文字 (PDF 提取):

Layering tissues
Build-up tissues layer 
by layer 
Grow perfused tubules
in co-culture 
with tissue in ECM
Follow LIVE 
migration/invasion 
in a horizontal plane
Cell migration 
& outgrowth
Not limited by a 
membrane 
or its pore size
Cells can freely 
migrate between layers 
Monitor cell function, 
differentiation, and 
proliferation
Cell-cell interaction
​Close proximity of cells
allows juxtracrine 
& paracrine signaling​
Retain local signaling 
factors in ECM​
No dilution of 
local signaling factors 
and analytes in large 
medium volumes​
No membrane 
interference
​No compensation needed
for ​pore size and
pore density
No internal optical 
controls needed​
Real-time imaging​
Culture without artificial membranes

第 24 页 / Page 24

📷 caption (中文翻译): PPT 第 24 页(全文内容见下方)。本页内容摘要: Pump-free perfusion Perfusion through passive leveling between wells of the plate Hundreds to thousands of chips in parallel No external pumps or tubing Automatable Allows studying influence on: • G

📄 原文文字 (PDF 提取):

Pump-free perfusion
Perfusion through passive leveling 
between wells of the plate
Hundreds to thousands of 
chips in parallel
No external pumps or tubing
Automatable
Allows studying influence on:
•
Gene expression and function
•
Ion channels & transporters
•
Cell morphology
Enhanced albumin uptake 
and P-gp activity by 
proximal tubule cells
Constant conditions 
& gradients
Rapid compound dosing
Homogeneous exposure conditions
Constant oxygen levels, removal of 
waste metabolites
Stable gradients
Trigger angiogenesis, polarization, 
migration and directed outgrowth
Shear stress
Perfused cultures in one go

第 25 页 / Page 25

📷 caption (中文翻译): PPT 第 25 页(全文内容见下方)。本页内容摘要: Unsurpassed 3D imaging 150𝜇m thin glass allows highest quality optical access Phase contrast, fluorescence & confocal imaging Time-lapse studies, high quality imaging, and 3D visualization Any r

📄 原文文字 (PDF 提取):

Unsurpassed 
3D imaging
150𝜇m thin glass allows 
highest quality optical 
access
Phase contrast, 
fluorescence & confocal 
imaging
Time-lapse studies,
high quality imaging, and
3D visualization 
Any readout
Fluorescence- , 
phase contrast imaging, 
plate readers
Gene expression analysis, 
ELISA, LC-MS
OrganoTEER®: 
high throughput TEER 
measurement
Compatible
Microtiter plate format 
compatible with any 
microscope, plate reader 
and robot
40-96 chips per plate for 
sufficient replicates, 
dilution series, 
and controls
Automatable
Fully compatible with 
automated imaging and 
robotic liquid handling 
Integration in a high 
throughput screening 
setup
Imaging, throughput & compatibility

第 26 页 / Page 26

📷 caption (中文翻译): PPT 第 26 页(全文内容见下方)。本页内容摘要: • Polarized tubular tissues against extracellular matrix (ECM) without artificial membranes • Perfusion for shear stress & homogeneous culture conditions • Enables permeability, absorption and transpo

📄 原文文字 (PDF 提取):

•
Polarized tubular tissues against extracellular matrix (ECM) without artificial membranes
•
Perfusion for shear stress & homogeneous culture conditions
•
Enables permeability, absorption and transport, transmigration, 
and transcytosis studies on e.g. intestine, kidney, retina, lung, skin, and blood vessel
Caco-2 intestinal tubule against ECM
Permeability / transport
ZO1 Ezrin DNA

第 27 页 / Page 27

📷 caption (中文翻译): PPT 第 27 页(全文内容见下方)。本页内容摘要: 1 Add ECM gel to the middle channel 2 Seed cells and grow a tubule 3 Select your assay Observe in real-time 4 Permeability study in 4 steps OrganoTEER® Fluorescent marker OrganoTEER® Fluorescent ma

📄 原文文字 (PDF 提取):

1
Add ECM gel 
to the middle channel
2
Seed cells and 
grow a tubule 
3
Select your assay
Observe in real-time
4
Permeability study in 4 steps
OrganoTEER®
Fluorescent marker
OrganoTEER®
Fluorescent marker

第 28 页 / Page 28

📷 caption (中文翻译): PPT 第 28 页(全文内容见下方)。本页内容摘要: Example of 3D cell culture modality to investigate hallmarks of inflammatory bowel disease (IBD): • Induction of inflammatory conditions caused loss of barrier function and activation • Loss of barr

📄 原文文字 (PDF 提取):

Example of 3D cell culture modality to investigate 
hallmarks of inflammatory bowel disease (IBD):
•
Induction of inflammatory conditions caused loss 
of barrier function and activation
•
Loss of barrier function prevented by exposure to 
anti-inflammatory compounds
•
Knockdown of key inflammatory regulators (RELA / 
MYD88) alleviated IBD phenotype by decreasing 
cytokine production
Development of a Gut-on-a-Chip Model for High Throughput Disease Modeling and Drug Discovery. Int. J. 
Mol. Sci. 2019, 20(22), 5661
Get inspired: 1) Intestinal model for IBD 
Effect of short and long cytokine trigger on morphology and 
integrity of Caco-2 tubules
TPCA-1 exposure decreases 
cytokine secretion of Caco-2 
tubules in a dose-dependent 
manner

第 29 页 / Page 29

📷 caption (中文翻译): PPT 第 29 页(全文内容见下方)。本页内容摘要: Establishment of a multicellular intestine-on-a-chip model • Unique robust, high-throughput 3D intestine-on-a- chip model with biological modularity to study intestinal inflammation • Multicellular

📄 原文文字 (PDF 提取):

Establishment of a multicellular intestine-on-a-chip 
model
•
Unique robust, high-throughput 3D intestine-on-a-
chip model with biological modularity to study 
intestinal inflammation
•
Multicellular culture to better recapitulate the 
intestinal physiology,  increase complexity, and 
introduce cell-cell interactions
•
Suitable for studying intestinal inflammation or 
screening anti-inflammatory compounds
Tubular epithelial structure of Caco-2 and HT29- MTX-E12 cells against a collagen I ECM 
patterned in the middle channel of OrganoPlate® 3-lane.
2) Multicellular intestinal model for IBD

第 30 页 / Page 30

📷 caption (中文翻译): IBD 炎症性肠病 4 细胞共培养 + TEER 验证页。4 细胞共培养: Caco-2 + HT29-MTX-E12 + THP-1 + MUTZ-3。TNFα/IL1β 炎症诱导 72h TEER 降至 55%,TPCA-1 阳性对照恢复至 80%。

📄 原文文字 (PDF 提取):

Case study 1: IBD disease model and assays
Goal: Development of an IBD model to assess immune-modulatory properties of compounds
Approach:
•
Setup a relevant, immune competent intestinal model in the OrganoPlate® platform
•
Extensive characterization of phenotype (marker expression, barrier function, cytokine release) 
•
Validation of IBD-phenotype using known disease modulating compounds
Assay:
Result: Unique IBD model with robust assays for drug screening and compound profiling 
Co-culture model of epithelial tubules, 
goblet cells, and immune cells
THP-1
MUTZ-3
TNFα
IL1β
Caco-2
HT29-MTX-
E12
Prevention of TNFa/IL1b-induced gut permeability 
measured by OrganoTEER®

第 31 页 / Page 31

📷 caption (中文翻译): PPT 第 31 页(全文内容见下方)。本页内容摘要: Detailed protocol for establishing and characterizing kidney tubuloids derived from kidney tissue or urine and for generating 3D structures in an organ on-a-chip system promoting: • Better physiolo

📄 原文文字 (PDF 提取):

Detailed protocol for establishing and characterizing kidney 
tubuloids derived from kidney tissue or urine and for 
generating 3D structures in an organ on-a-chip system 
promoting:
•
Better physiological tubular architecture that more closely 
represent the in vivo kidney
•
Induction of flow-induced shear stress
•
Increasing model complexity
•
Real-time readout of leak-tightness and transport
The combination of tubuloids with organ-on-a-chip systems 
yields a powerful in vitro tool that better recapitulates the 
complexity of the kidney tubule with donor-specific properties.
90
Tubuloid cells on-a-chip in the OrganoPlate® 3-Lane. A) 3D reconstruction of 
a confocal z-stack showing a polarized  tubular morphology with a lumen 
against the ECM gel. B) 35 leak-tight tubuloid on-a-chip cultures and 5 no cell 
controls (first column). Scale bars = 200 µm.  
3) Nature protocol: Establishing kidney tubuloids on-a-chip
A
B

第 32 页 / Page 32

📷 caption (中文翻译): PPT 第 32 页(全文内容见下方)。本页内容摘要: Better intestinal barriers without plate inserts

📄 原文文字 (PDF 提取):

Better intestinal barriers without plate inserts

第 33 页 / Page 33

📷 caption (中文翻译): PPT 第 33 页(全文内容见下方)。本页内容摘要: Study towards disease induction in a human glomerular filtration model:​ • Deposition of de novo extracellular membrane components that are specific to the glomerulus • Confirmed perm selectivity to

📄 原文文字 (PDF 提取):

Study towards disease induction in a 
human glomerular filtration model:​
•
Deposition of de novo extracellular
membrane components that are specific 
to the glomerulus
•
Confirmed perm selectivity to albumin
•
Serum of patients with membranous
nephropathy induces loss of barrier
function that is correlated to clinical findings​
A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Com. 10, 
3656 (2019).
Get inspired: 3D glomerulus model
Serum of patients with membranous nephropathy induce albumin 
leakage in vitro that correlates with protein levels in urea
Glomerulus barrier constituted of podocytes (NPHS1) and glomerular 
endothelium (CD31)

第 34 页 / Page 34

📷 caption (中文翻译): PPT 第 34 页(全文内容见下方)。本页内容摘要: • Co-culture different cell types without artificial membranes​ • Enable stratified co-cultures with relevant tissue structures​ • Study cellular interactions, cell-cell signaling, migration Neurovasc

📄 原文文字 (PDF 提取):

•
Co-culture different cell types without artificial membranes​
•
Enable stratified co-cultures with relevant tissue structures​
•
Study cellular interactions, cell-cell signaling, migration
Neurovascular unit: co-culture of human brain endothelial cells, iPSC-derived neurons and astrocytes
Cell-cell interactions
HUVEC-RFP 
endothelium
CD44 
astrocytes
MAP2 
neurons

第 35 页 / Page 35

📷 caption (中文翻译): Angiogenesis 血管生成 HUVEC 页。HUVEC 在 OrganoPlate 形成三维微血管,可稳定维持 4+ 天。3 色荧光通道: 蓝色 DNA + 绿色 VE Cadherin + 红色 Actin。Tip-stalk hierarchy + anastomosis。

📄 原文文字 (PDF 提取):

•
Perfused 3D microvasculature​
•
Stable capillary structure for 4+ days​
•
Tip-stalk cell hierarchy & anastomosis​
•
Investigate microvascular damage, tumor angiogenesis, fibrosis​
Culture of HUVECs forming angiogenic sprouts from pre-existing vessel
Angiogenesis
DNA 
VE Cadherin 
Actin

第 36 页 / Page 36

📷 caption (中文翻译): PPT 第 36 页(全文内容见下方)。本页内容摘要: 1 Grow a perfused blood vessel 3 Observe angiogenesis 2 Add angiogenic cocktail to form angiogenic gradient Angiogenesis in 3 steps

📄 原文文字 (PDF 提取):

1
Grow a perfused 
blood vessel
3
Observe 
angiogenesis
2
Add angiogenic 
cocktail to form 
angiogenic gradient
Angiogenesis in 3 steps

第 37 页 / Page 37

📷 caption (中文翻译): PPT 第 37 页(全文内容见下方)。本页内容摘要: Study towards inhibition of angiogenesis in robust and screenable model: • Induce angiogenesis from HUVEC and iPSC derived endothelial cell (EC) vessel • Stable sprouts formation with lumen, tip-sta

📄 原文文字 (PDF 提取):

Study towards inhibition of angiogenesis in robust and 
screenable model:
•
Induce angiogenesis from HUVEC and iPSC derived 
endothelial cell (EC) vessel
•
Stable sprouts formation with lumen, tip-stalk cells, 
neovessel perfusion and anastomosis
•
Exposing micro vessels to sprouting inhibitors sunitinib and 
3PO, significantly reduced sprouting of both primary and 
iPSC-derived Ecs
•
Suitable for screening of anti-angiogenic compounds (Z’ 0,75)
Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived 
Endothelium for Anti-Angiogenic Drug Screening. Int. J. Mol. Sci. 2020, 21, 4804.
Get inspired: angiogenesis model
iPSC-derived endothelial 
tubes are exposed to 
increasing concentrations to 
sunitib in combination with a 
cocktail of angiogenic factors

第 38 页 / Page 38

📷 caption (中文翻译): PPT 第 38 页(全文内容见下方)。本页内容摘要: In vitro vascularization is the missing link in cell- and tissue culture • In vitro grafting of target tissues on a vascular bed​ • Study interaction between perfused vasculature & target tissues​ •

📄 原文文字 (PDF 提取):

In vitro vascularization is the missing link in cell- and tissue 
culture
•
In vitro grafting of target tissues on a vascular bed​
•
Study interaction between perfused vasculature & target 
tissues​
•
Spheroids, organoids, tissue fragments
•
Patient-derived tissue grafting in vitro: 
an alternative to PDX mice
Vascularized liver spheroid in the 
OrganoPlate® Graft
Vascularization

第 39 页 / Page 39

📷 caption (中文翻译): PPT 第 39 页(全文内容见下方)。本页内容摘要: 1 ECM filling Graft chamber 2 Seeding endothelium 3 Microvascular bed formation Co-culture with target tissue 4 Vascularization in 4 steps OrganoPlate® Graft has an open-top design ​to receive tar

📄 原文文字 (PDF 提取):

1
ECM filling
Graft 
chamber
2
Seeding 
endothelium
3
Microvascular 
bed formation
Co-culture with 
target tissue
4
Vascularization in 4 steps
OrganoPlate® Graft has an open-top design ​to receive target tissues for studying interaction ​with a vascular bed.

第 40 页 / Page 40

📷 caption (中文翻译): 血管化肝脏类器官 5 研究图页。CD31+ 血管网络长入肝球,密闭性 OK。HIF1A -70%,CYP3A4 +6 倍,长期 LDH 维持基线。5 项核心结论验证血管化对肝功能的提升。

📄 原文文字 (PDF 提取):

Study of in vitro vascularization of liver organoids, 
shows:
•
Infiltration of vessels into liver organoids​
•
Vessels become leaktight upon connecting
(anastamosis)
•
Constant albumin levels​
•
Reduced hypoxia-related stress in
vascularized spheroids
•
Vascularization increases metabolic activity and 
maturation of the hepatic organoids
•
Prolonged homeostasis in vascularized hepatic 
organoids
Get inspired: vascularized liver model
Albumin CD31
Connected
No organoid
L D H re le a s e fro m d a m a g e d c e lls
L D H fo ld re le a s e
(re la tiv e to d a y 7 )
He p atic o r g an o id s 
M icr o ve s s e ls 
He p aic o r g an o id s + m icr o ve s s e ls
0.0
0.5
1.0
1.5
2.0
2.5
3.0
D a y 7
D a y 1 4
D a y 2 1
H e p a tic
o rg a n o id s
m ic ro v e s s e ls
H e p a tic o r g a n o id s
+ m ic ro v e s s e ls
****
****
C Y P 3 A 4 e x p re s s io n
F o ld e x p re s s io n
re la tiv e to H e p a tic o rg a n o id s
n o t e xp o se d
e xp o se d AP AP [10 m M ]
0
5
10
15
H e p atic org a no ids
H ep atic orga no id s+ m icrovesse ls
m R N A H IF1A
2 ^ C t
(re la tiv e to s p h e ro id s )
0.0
0.5
1.0
1.5
s p h e ro id s
s p h e ro id s
+ m ic ro v e s s e ls
m ic r o v e s s e ls

第 41 页 / Page 41

📷 caption (中文翻译): PPT 第 41 页(全文内容见下方)。本页内容摘要: • OrganoPlate Graft • Co-culture of lung epithelium with vascular network • Air-liquid interface (ALI) 100 Strictly confidential Lung-on-a-chip

📄 原文文字 (PDF 提取):

•
OrganoPlate Graft
•
Co-culture of lung 
epithelium with vascular 
network
•
Air-liquid interface (ALI)
100
Strictly confidential
Lung-on-a-chip

第 42 页 / Page 42

📷 caption (中文翻译): PPT 第 42 页(全文内容见下方)。本页内容摘要: Quality control of epithelial tubules • Increased barrier formation over time • Stable TEER values from day 4 (Caco-2) OrganoTEER® application examples Caco-2 over time Time days TEER ohm*cm2 ZO-1

📄 原文文字 (PDF 提取):

Quality control of epithelial tubules
•
Increased barrier formation over time
•
Stable TEER values from day 4 (Caco-2)
OrganoTEER® application examples
Caco-2 over time
Time [days]
TEER [ohm*cm2]
ZO-1 (tight junction marker) in green, nuclei in blue

第 43 页 / Page 43

📷 caption (中文翻译): PPT 第 43 页(全文内容见下方)。本页内容摘要: Disease modeling • Cytokine exposure to Caco-2 tubules reduces barrier function as shown by reduced TEER values • Addition of TPCA-1 prevents loss of barrier function OrganoTEER® application exampl

📄 原文文字 (PDF 提取):

Disease modeling
•
Cytokine exposure to Caco-2 tubules reduces 
barrier function as shown by reduced TEER 
values
•
Addition of TPCA-1 prevents loss of barrier 
function
OrganoTEER® application examples
Caco-2
T E E R : C a c o -2 tu b e s
 . c m 2
C T R
c y to k in e s 1 0 0 n g /m l
0
1 0 0
2 0 0
3 0 0
4 0 0
5 0 0
n = 1 6
Caco-2/
HT-29 tube
Differentiated
THP-1
T E E R : C a c o -2 /H T 2 9 T u b e
T E E R V a lu e  (O h m s  * c m 2)
N o T re a tm e n t 
IL -1 3 + T N F a + IL 1 B
IL -1 3 + T N F a + IL 1 B + D A P T
IL -1 3 + T N F a + IL 1 B + T P C A -1
0
1 0 0
2 0 0
3 0 0
4 0 0
5 0 0
N o T reatm ent
IL -1 3 + T N F a + IL 1 B
IL -1 3 + T N F a + IL 1 B + D A P T
IL -1 3 + T N F a + IL 1 B + T P C A -1
All n=4
Model disease phenotype (IBD) 
Drug screening: rescue IBD phenotype

第 44 页 / Page 44

📷 caption (中文翻译): PPT 第 44 页(全文内容见下方)。本页内容摘要: Supporting low TEER measurements • Sensitive low TEER measurements • Possibility to measure dose-dependent effect of toxicants on endothelial cells​ • Successful measurement with a variety of cell lin

📄 原文文字 (PDF 提取):

Supporting low TEER measurements
•
Sensitive low TEER measurements
•
Possibility to measure dose-dependent effect of toxicants on endothelial cells​
•
Successful measurement with a variety of cell lines

第 45 页 / Page 45

📷 caption (中文翻译): 低 TEER 测量验证页。HUVEC + Staurosporine 梯度 11/33/100/300nM,23h 动态 TEER。300nM 1.5h 跌至 ~0,OrganoTEER 在 0~5 Ω·cm² 极低电阻区间验证精度。

📄 原文文字 (PDF 提取):

Supporting low TEER measurements
•
Clear dose dependent effect of 
Staurosporine detected with 
OrganoTEER
•
Top graph showing TEER values
•
Bottom graph showing normalized 
TEER data

💼 合作模式与服务

• In our labs: Mimetas 实验室合作开发
• Custom model & assay development: 定制化模型与检测开发(2-5 天专属项目)
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• Starter kits: 入门套装(OrganoStart Pro Package)

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🏷️ 竞品定位 (国际竞品,非代理)

反模式提醒:

• ❌ 不写"Mimetas 是已代理产品"
• ✅ 写"国际竞品,内部拍板确认非代理关系"

📖 资料信息

📥 原文 PDF 下载

• 联系人:李东伟
• 📞 18045130660
• 🌐 https://www.biokingdom.top

转发语: 🧠⚡ Mimetas 2024 客户演示 PPT 中文版 (45 页) 完整图文存档 v3:全 45 页每页 1 图 + 原文文字 + 中文 caption,从 PPT 封面 (p.1) 到应用案例 (p.45) 全还原。OrganoPlate / OrganoFlow / OrganoTEER 三大产品线 + 6 大应用方向 + 4 大案例 (IBD / HUVEC / 血管化肝球 / 低 TEER) + 4 类合作模式 + 国际竞品定位,实战资料存档。